PTP1B/PTPN1 Antibody (M234)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23189

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # M234

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PTP1B Antibodies »

Product Summary for PTP1B/PTPN1 Antibody (M234)

Immunogen

Clone M234 was generated from a recombinant protein corresponding to amino acids in the C-terminal region of human PTP1B/PTPN1. [UniProt# P18031]

Specificity

This antibody detects a 50 kDa band corresponding to PTP1B/PTPN1 in western blots of human A431, Jurkat, and HeLa cells. It does not detect PTP1B/PTPN1 in rat PC12 or mouse SYF cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for PTP1B/PTPN1 Antibody (M234)

PTP1B/PTPN1 Antibody (M234)

Western Blot: PTP1B/PTPN1 Antibody (M234) [NBP3-23189] -

Western Blot: PTP1B/PTPN1 Antibody (M234) [NBP3-23189] - Western blot image of human Jurkat cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with calyculin A (100 nM for 30 min.). The blots were probed with mouse monoclonal anti-PTP1B/PTPN1 (lanes 1 & 2) or rabbit polyclonal anti-PTP1B/PTPN1 (Ser-50) (lanes 3 & 4).
PTP1B/PTPN1 Antibody (M234)

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (M234) [NBP3-23189] -

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (M234) [NBP3-23189] - Immunocytochemical labeling of PTP1B/PTPN1 in methanol and acetone fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monclonal anti-PTP1B/PTPN1 (NBP3-23189) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Applications for PTP1B/PTPN1 Antibody (M234)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PTP1B

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Gene Symbol

PTPN1

Additional PTP1B Products

Product Documents for PTP1B/PTPN1 Antibody (M234)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PTP1B/PTPN1 Antibody (M234)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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