Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PTPN23 Antibodies »

Product Summary for PTPN23 Antibody

Immunogen

The immunogen recognized by the antibody maps to a region between residue 1586 to 1636 of human HisDomain Protein Tyrosine Phosphatase using the numbering given in entry NP_056281.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTPN23 Antibody

Western Blot: PTPN23 Antibody [NBP2-36549]

Western Blot: PTPN23 Antibody [NBP2-36549]

Western Blot: PTPN23 Antibody [NBP2-36549] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HD-PTP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PTPN23 Antibody [NBP2-36549]

Immunoprecipitation: PTPN23 Antibody [NBP2-36549]

Immunoprecipitation: PTPN23 Antibody [NBP2-36549] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. For blotting immunoprecipitated HD-PTP, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PTPN23 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PTPN23

PTPN23 may act as a negative regulator of Ras-mediated mitogenic activity

Long Name

Tyrosine-protein phosphatase non-receptor type 23

Alternate Names

HD-PTP, KIAA1471, PTP-TD14

Gene Symbol

PTPN23

UniProt

NP_056281.1 (Human)

Additional PTPN23 Products

Product Documents for PTPN23 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PTPN23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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