Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3B10

Concentration

0.72 mg/ml

View all PTPN7 Antibodies »

Product Summary for PTPN7 Antibody (OTI3B10)

Immunogen

Full length human recombinant protein of human PTPN7(NP_542155) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

44.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTPN7 Antibody (OTI3B10)

Western Blot: PTPN7 Antibody (3B10) [NBP2-01340] Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN7 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PTPN7 Antibody (OTI3B10) [NBP2-01340]

Immunocytochemistry/ Immunofluorescence: PTPN7 Antibody (OTI3B10) [NBP2-01340]

Immunocytochemistry/Immunofluorescence: PTPN7 Antibody (3B10) [NBP2-01340] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN7.
Western Blot: PTPN7 Antibody (OTI3B10) [NBP2-01340]

Western Blot: PTPN7 Antibody (OTI3B10) [NBP2-01340]

Western Blot: PTPN7 Antibody (3B10) [NBP2-01340] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN7.

Applications for PTPN7 Antibody (OTI3B10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-01340 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.72 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTPN7

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

Long Name

Tyrosine-protein phosphatase non-receptor type 7

Alternate Names

BPTP-4, FLJ18703, HEPTPnonreceptor-type, stress induced, LPTP, protein tyrosine phosphatase, non-receptor type 7, tyrosine-protein phosphatase non-receptor type 7

Entrez Gene IDs

5778 (Human)

Gene Symbol

PTPN7

Additional PTPN7 Products

Product Documents for PTPN7 Antibody (OTI3B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PTPN7 Antibody (OTI3B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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