PU.1/Spi-1 Antibody (S07-4D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14966

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14966-100ul
NBP3-14966-25ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-4D3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PU.1/Spi-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human PU.1/Spi-1 (Uniprot#: P17947)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PU.1/Spi-1 Antibody (S07-4D3)

Western Blot: PU.1/Spi-1 Antibody (S07-4D3) [NBP3-14966]

Western Blot: PU.1/Spi-1 Antibody (S07-4D3) [NBP3-14966]

Western Blot: PU.1/Spi-1 Antibody (S07-4D3) [NBP3-14966] - Western blot detection of PU. 1/Spi-1 in Daudi, mouse liver cell lysates using PU. 1/Spi-1 Rabbit mAb (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 42kDa.

Applications for PU.1/Spi-1 Antibody (S07-4D3)

Application
Recommended Usage

Western Blot

1/1000-1/2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PU.1/Spi-1

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody (S07-4D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PU.1/Spi-1 Antibody (S07-4D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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