PU.1/Spi-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00135

Novus Biologicals, part of Bio-Techne
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NBP1-00135
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PU.1/Spi-1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DLYQRQTHEYY corresponding to internal region according to NP_001074016.1, NP_003111.2.

Epitope

internal region

Specificity

This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PU.1/Spi-1 Antibody

Western Blot: PU.1/Spi-1 Antibody [NBP1-00135]

Western Blot: PU.1/Spi-1 Antibody [NBP1-00135]

Western Blot: PU.1/Spi-1 Antibody [NBP1-00135] - (0.03ug/ml) Analysis of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135]

Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135]

Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135]

Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135] - Analysis of PU.1/Spi1 in p3xFLAG-PU.1 transfected 293T cells (1 million cells) using anti-PU.1/Spi1 antibody. Image from verified customer review.

Applications for PU.1/Spi-1 Antibody

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in lysates of DAUDI and K562 is reported in scientific literature (PMID: 10867017).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-00135 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PU.1/Spi-1

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Entrez Gene IDs

6688 (Human); 366126 (Rat)

Gene Symbol

SPI1

UniProt

NP_001074016.1 (Human)

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PU.1/Spi-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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