PU.1/Spi-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27163

Novus Biologicals, part of Bio-Techne
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NBP2-27163
NBP2-27163SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Bovine, Canine, Equine, Primate

Predicted:

Chimpanzee (100%), Monkey (100%), Porcine (100%), Rat (92%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all PU.1/Spi-1 Antibodies »

Product Specifications

Immunogen

A portion of amino acid 20-70 of human PU.1 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 89% homologous in Opossum.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PU.1/Spi-1 Antibody

Western Blot: PU.1/Spi-1 Antibody [NBP2-27163]

Western Blot: PU.1/Spi-1 Antibody [NBP2-27163]

Western Blot: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Analysis of PU.1/Spi1 in U937 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27163.
Flow Cytometry: PU.1/Spi-1 Antibody [NBP2-27163]

Flow Cytometry: PU.1/Spi-1 Antibody [NBP2-27163]

Flow Cytometry: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Intracellular analysis of PU.1 in mouse RAW cells (top) and PMA treated (20 ng/ml, overnight) human ThP1 cells (bottom) using 1.25 ug/10^6 cells. Green peak represents isotype control this antibody ; red represents anti-PU.1 antibody.
Western Blot: PU.1/Spi-1 Antibody [NBP2-27163]

Western Blot: PU.1/Spi-1 Antibody [NBP2-27163]

Western Blot: PU.1/Spi1 Antibody [NBP2-27163] - analysis of 293T cell lysate (30ug) using anti-PU.1/Spi1 antibody. Image from verified customer review.

Applications for PU.1/Spi-1 Antibody

Application
Recommended Usage

Flow Cytometry

1.25 ug/10^6 cells

Western Blot

4-6ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-27163 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PU.1/Spi-1

Transcription factor PU.1 belongs to the ETS family and contains one ETS DNA-binding domain. It is a novel caspase-3 substrate and found to contribute partially chemotherapeutic agents-induced apoptosis in leukemic cellsThis protein is also reported to activate gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. It is found to be one of the key regulators of hematopoitic cell development and induces apoptosis in myeloma cells through direct transactivation of an apoptosis-related gene, tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL).

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

UniProt

P17947 (Human)

Additional PU.1/Spi-1 Products

Product Documents for PU.1/Spi-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PU.1/Spi-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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