PUF60 Antibody (1C1)

Novus Biologicals, part of Bio-Techne | Catalog # H00022827-M20

Novus Biologicals, part of Bio-Techne
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H00022827-M20
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PUF60 Antibodies »

Product Specifications

Immunogen

PUF60 (AAH11979, 1 a.a. ~ 516 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Specificity

Reacts with poly-U binding splicing factor 60KDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PUF60 Antibody (1C1)

Western Blot: PUF60 Antibody (1C1) [H00022827-M20]

Western Blot: PUF60 Antibody (1C1) [H00022827-M20]

Western Blot: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody (M20), clone 1C1. Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PUF60 Antibody (1C1) [H00022827-M20]

Immunocytochemistry/ Immunofluorescence: PUF60 Antibody (1C1) [H00022827-M20]

Immunocytochemistry/Immunofluorescence: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of monoclonal antibody to PUF60 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: PUF60 Antibody (1C1) [H00022827-M20] - Detection limit for recombinant GST tagged PUF60 is 0.1 ng/ml as a capture antibody.

Applications for PUF60 Antibody (1C1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PUF60

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]

Alternate Names

FBP interacting repressor, FBP-interacting repressor, FIRpoly-U binding splicing factor PUF60, FLJ31379, FUSE-binding protein-interacting repressor, poly(U)-binding-splicing factor PUF60, poly-U binding splicing factor 60KDa, Ro ribonucleoprotein binding protein 1, Ro ribonucleoprotein-binding protein 1, Ro-binding protein 1, roBP1, ROBPI, siah binding protein 1,60 kDa poly(U)-binding-splicing factor, Siah-binding protein 1, Siah-BP1, SIAHBP1pyrimidine tract binding splicing factor

Entrez Gene IDs

22827 (Human)

Gene Symbol

PUF60

UniProt

AAH11979 (Human)

Additional PUF60 Products

Product Documents for PUF60 Antibody (1C1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUF60 Antibody (1C1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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