PUM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87856

Novus Biologicals, part of Bio-Techne
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NBP1-87856
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PUM1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTNGLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PUM1 Antibody

Western Blot: PUM1 Antibody [NBP1-87856]

Western Blot: PUM1 Antibody [NBP1-87856]

Western Blot: PUM1 Antibody [NBP1-87856] - Analysis using Anti-PUM1 antibody NBP1-87856 (A) shows similar pattern to independent antibody NBP2-58337 (B).
Immunocytochemistry/ Immunofluorescence: PUM1 Antibody [NBP1-87856]

Immunocytochemistry/ Immunofluorescence: PUM1 Antibody [NBP1-87856]

Immunocytochemistry/Immunofluorescence: PUM1 Antibody [NBP1-87856] - Staining of human cell line U-251 MG shows positivity in cytoplasm.
Immunohistochemistry-Paraffin: PUM1 Antibody [NBP1-87856]

Immunohistochemistry-Paraffin: PUM1 Antibody [NBP1-87856]

Immunohistochemistry-Paraffin: PUM1 Antibody [NBP1-87856] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
View 1 more images

Applications for PUM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUM1

Pumilio-1 encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Long Name

Pumilio 1

Alternate Names

HSPUM, PUMH1, Pumilio-1, PUML1

Gene Symbol

PUM1

Additional PUM1 Products

Product Documents for PUM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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