Skip to main content

PUM2 Antibody (JE37-76)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32879

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-32879

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE37-76

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within Human PUM2 aa 1-100. (Uniprot: Q8TB72)

Localization

Cytoplasm, Cytoplasmic granule, perinuclear region.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PUM2 Antibody (JE37-76)

PUM2 Antibody (JE37-76)

Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879]

Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879] - Western blot analysis of PUM2 on different lysates with Rabbit anti-PUM2 antibody (NBP3-32879) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: MCF7 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 114 kDa
Observed band size: 140 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32879) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
PUM2 Antibody (JE37-76)

Flow Cytometry: PUM2 Antibody (JE37-76) [NBP3-32879]

Flow Cytometry: PUM2 Antibody (JE37-76) [NBP3-32879] - Flow cytometric analysis of U-2 OS cells labeling PUM2.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32879, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications for PUM2 Antibody (JE37-76)

Application
Recommended Usage

Flow Cytometry

1:500-1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUM2

Pumilio 2 is a component of conserved cellular machinery required for germ cell development, Pum2 is a human homolog of Pumilio, which helps maintain germ line stem cells in Drosophila and C. elegans. Pum2 forms a stable complex with DAZ through the same functional domain required for RNA binding and protein-protein interactions. Pum2 is expressed predominantly in human embryonic stem cells and germ cells and colocalizes with DAZ and DAZL in germ cells.

Long Name

Pumilio 2

Alternate Names

PUMH2, Pumilio-2, PUML2

Gene Symbol

PUM2

Additional PUM2 Products

Product Documents for PUM2 Antibody (JE37-76)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PUM2 Antibody (JE37-76)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...