PUM2 Antibody (JE37-76)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32879
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE37-76
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human PUM2 aa 1-100. (Uniprot: Q8TB72)
Localization
Cytoplasm, Cytoplasmic granule, perinuclear region.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PUM2 Antibody (JE37-76)
Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879]
Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879] - Western blot analysis of PUM2 on different lysates with Rabbit anti-PUM2 antibody (NBP3-32879) at 1/1,000 dilution.Lane 1: A549 cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: MCF7 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 114 kDa
Observed band size: 140 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32879) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Flow Cytometry: PUM2 Antibody (JE37-76) [NBP3-32879]
Flow Cytometry: PUM2 Antibody (JE37-76) [NBP3-32879] - Flow cytometric analysis of U-2 OS cells labeling PUM2.Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32879, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Applications for PUM2 Antibody (JE37-76)
Application
Recommended Usage
Flow Cytometry
1:500-1:1000
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PUM2
Long Name
Pumilio 2
Alternate Names
PUMH2, Pumilio-2, PUML2
Gene Symbol
PUM2
Additional PUM2 Products
Product Documents for PUM2 Antibody (JE37-76)
Product Specific Notices for PUM2 Antibody (JE37-76)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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