PUM2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-387
![Western Blot: PUM2 Antibody [NB100-387]](https://resources.bio-techne.com/images/products/PUM2-Antibody-Western-Blot-NB100-387-img0003.jpg "Western Blot: PUM2 Antibody [NB100-387]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Pumilio 2 using the numbering given in entry NP_056132.1 (GeneID 23369).
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 25097251).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PUM2 Antibody
Western Blot: PUM2 Antibody [NB100-387]
Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY license.![Western Blot: PUM2 Antibody [NB100-387]](https://resources.bio-techne.com/images/products/PUM2-Antibody-Western-Blot-NB100-387-img0002.jpg "Western Blot: PUM2 Antibody [NB100-387]")
Western Blot: PUM2 Antibody [NB100-387]
Western Blot: PUM2 Antibody [NB100-387] - Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody NB100-387 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.Applications for PUM2 Antibody
Application
Recommended Usage
Immunoprecipitation
5-10 ug/mg lysate
Western Blot
1:10000-1:20000
Application Notes
By Western blot, this antibody recognizes bands at ~135-160 kDa, representing Pum2. A non-specific band at ~40 kDa may be seen in mouse lysates and just above 250 kDa in human lysates.
Reviewed Applications
Read 1 review rated 4 using NB100-387 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PUM2
Long Name
Pumilio 2
Alternate Names
PUMH2, Pumilio-2, PUML2
Gene Symbol
PUM2
UniProt
Additional PUM2 Products
Product Documents for PUM2 Antibody
Product Specific Notices for PUM2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...