PUMA Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56370
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 186-199 (PLPRGHRAPEMEPN) of human PUMA a was used as the immunogen.
Reactivity Notes
The amino acid sequence used as immunogen is 78% homologous in rat.
Specificity
The amino acid sequence used as immunogen is 100% homologous in human (PUMA a and b). Human PUMA a is a 193 amino acid protein with a predicted molecular weight of approximately 22 kD. Human PUMA b is a 131 amino acid protein with a predicted molecular weight of approximately 15 kD.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PUMA Antibody - BSA Free
![Western Blot: PUMA Antibody [NB100-56370]](https://resources.bio-techne.com/images/products/PUMA-Antibody-Western-Blot-NB100-56370-img0003.jpg "Western Blot: PUMA Antibody [NB100-56370]")
Western Blot: PUMA Antibody [NB100-56370]
Western Blot: PUMA Antibody [NB100-56370] - Analysis for Puma a (CT) using NB100-56370 at 1:500 against 30 ug/lane of A) Jurkat and B) NIH 3T3 whole cell lysate.![Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]](https://resources.bio-techne.com/images/products/PUMA-Antibody-Immunohistochemistry-Paraffin-NB100-56370-img0005.jpg "Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]")
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells.![Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]](https://resources.bio-techne.com/images/products/PUMA-Antibody-Immunohistochemistry-Paraffin-NB100-56370-img0004.jpg "Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]")
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells. The representative section is showing alveolar area only.Applications for PUMA Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:250
Immunohistochemistry-Paraffin
reported in scientific literature (Karst et al (2005))
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PUMA
Alternate Names
BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994
Gene Symbol
BBC3
Additional PUMA Products
Product Documents for PUMA Antibody - BSA Free
Product Specific Notices for PUMA Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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