PUMA Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56370
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 186-199 (PLPRGHRAPEMEPN) of human PUMA a was used as the immunogen.
Reactivity Notes
The amino acid sequence used as immunogen is 78% homologous in rat.
Specificity
The amino acid sequence used as immunogen is 100% homologous in human (PUMA a and b). Human PUMA a is a 193 amino acid protein with a predicted molecular weight of approximately 22 kD. Human PUMA b is a 131 amino acid protein with a predicted molecular weight of approximately 15 kD.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PUMA Antibody
![Western Blot: PUMA Antibody [NB100-56370]](https://resources.bio-techne.com/images/products/PUMA-Antibody-Western-Blot-NB100-56370-img0003.jpg "Western Blot: PUMA Antibody [NB100-56370]")
Western Blot: PUMA Antibody [NB100-56370]
Western Blot: PUMA Antibody [NB100-56370] - Analysis for Puma a (CT) using NB100-56370 at 1:500 against 30 ug/lane of A) Jurkat and B) NIH 3T3 whole cell lysate.![Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]](https://resources.bio-techne.com/images/products/PUMA-Antibody-Immunohistochemistry-Paraffin-NB100-56370-img0005.jpg "Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]")
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells.![Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]](https://resources.bio-techne.com/images/products/PUMA-Antibody-Immunohistochemistry-Paraffin-NB100-56370-img0004.jpg "Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]")
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370]
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells. The representative section is showing alveolar area only.Applications for PUMA Antibody
Application
Recommended Usage
Immunohistochemistry
1:50-1:250
Immunohistochemistry-Paraffin
reported in scientific literature (Karst et al (2005))
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PUMA
Alternate Names
BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994
Gene Symbol
BBC3
Additional PUMA Products
Product Documents for PUMA Antibody
Product Specific Notices for PUMA Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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