PUMA Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-230

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PUMA Antibodies »

Product Specifications

Immunogen

Reacts with a 17 residue sequence [EQHLESPVPSAPGALAG] found in the exon-3-encoded region in the human PUMA-alpha and PUMA-beta forms of the protein. Recognizes both forms of the protein.

Reactivity Notes

Reacts with human. 88% sequence identity with rat and mouse proteins.

Localization

Mitochondrial

Specificity

Human PUMA protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PUMA Antibody - BSA Free

Western Blot: PUMA AntibodyBSA Free [NB500-230]

Western Blot: PUMA AntibodyBSA Free [NB500-230]

Western Blot: PUMA Antibody [NB500-230] - WB detection of PUMA in transfected U20S cells using NB500-230 at a dilution of 1:250. Please note that this antibody is expected to detect alpha as well as the beta forms of this target.

Applications for PUMA Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:250 -1:1000
Application Notes
This antibody can be used in western blot, where the predicted molecular weight is 20 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUMA

A novel gene PUMA (p53 upregulated modulator of apoptosis) is a target for activation by the p53 tumor-suppressor gene. p53 functions as a transcriptional activator, and influences p53-inducible genes that play a role in the induction of apoptosis in response to p53. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

Alternate Names

BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994

Entrez Gene IDs

27113 (Human)

Gene Symbol

BBC3

Additional PUMA Products

Product Documents for PUMA Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUMA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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