PUMA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB500-261

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

5.65 mg/ml

View all PUMA Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a region within the C-terminus of the human PUMA protein sequence (between residues 150-193). [UniProt# Q9BXH1]

Localization

Mitochondrial

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PUMA Antibody

Western Blot: PUMA Antibody [NB500-261]

Western Blot: PUMA Antibody [NB500-261]

Western Blot: PUMA Antibody [NB500-261] - Detection of the beta isoform of PUMA in HL-60 whole cell lysate using NB 500-261(4 ug/ml). ECL detection at a 30 minute exposure.
Immunocytochemistry/ Immunofluorescence: PUMA Antibody [NB500-261]

Immunocytochemistry/ Immunofluorescence: PUMA Antibody [NB500-261]

Immunocytochemistry/Immunofluorescence: PUMA Antibody [NB500-261] - The PUMA antibody was tested at a 1:20 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

Applications for PUMA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Western Blot

2-4 ug/ml
Application Notes
This PUMA antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. By WB a band at ~16 kDa is seen, representing the beta form of PUMA. Reactivity against the alpha form of PUMA is unknown. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

5.65 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUMA

PUMA (p53 upregulated modulator of apoptosis) belongs to pro-apoptotic members of Bcl2 family's BH3-only subgroup, and besides its most critical role as mediator of p53/TP53-dependent/-independent apoptosis, it acts as a regulator of ER stress-induced neuronal apoptosis also. PUMA is expressed ubiquitously and predominately localizes to mitochondria where it execute induction of cytochrome c release. PUMA mediates apoptosis via direct binding with anti-apoptotic members of the Bcl-2 family and is capable of interacting with MCL1, BCL2A1, BCL2 and BCL2L1/BCL-XL. PUMA binding to inhibitory members of Bcl-2 family (Bcl-2-like proteins) leads to Bax and/or Bak displacement mediated activation and formation of pore-like structures over mitochondrial membrane, which permeabilizes the outer mitochondrial membrane, resulting in mitochondrial dysfunction and caspase activation. PUMA is induced by p53, DNA damage, glucocorticoid treatment, deprivation of growth factors etc. and through ER stress in a DDIT3/CHOP-dependent manner. PUMA has been reported to involve in multiple physiological and pathological processes, including immune response, cancer, neurodegenerative diseases and bacterial as well as viral infections.

Alternate Names

BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994

Gene Symbol

BBC3

Additional PUMA Products

Product Documents for PUMA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUMA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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