PURA Antibody (3F10)

Novus Biologicals, part of Bio-Techne | Catalog # H00005813-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PURA Antibodies »

Product Specifications

Immunogen

PURA (NP_005850, 183 a.a. ~ 292 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TQGQTIALPAQGLIEFRDALAKLIDDYGVEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWAKFGHTFCKYSEETKKIQEKQREKRAAC

Specificity

PURA - purine-rich element binding protein A (3F10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PURA Antibody (3F10)

Western Blot: PURA Antibody (3F10) [H00005813-M01]

Western Blot: PURA Antibody (3F10) [H00005813-M01]

Western Blot: PURA Antibody (3F10) [H00005813-M01] - PURA monoclonal antibody (M01), clone 3F10. Western Blot analysis of PURA expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: PURA Antibody (3F10) [H00005813-M01]

Immunocytochemistry/ Immunofluorescence: PURA Antibody (3F10) [H00005813-M01]

Immunocytochemistry/Immunofluorescence: PURA Antibody (3F10) [H00005813-M01] - Analysis of monoclonal antibody to PURA on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: PURA Antibody (3F10) [H00005813-M01]

Western Blot: PURA Antibody (3F10) [H00005813-M01]

Western Blot: PURA Antibody (3F10) [H00005813-M01] - PURA monoclonal antibody (M01), clone 3F10. Analysis of PURA expression in human skeletal muscle.
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Applications for PURA Antibody (3F10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against tissue lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PURA

This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.

Alternate Names

PUR1PUR-ALPHA, PURALPHA, purine-rich element binding protein A, Purine-rich single-stranded DNA-binding protein alpha, transcriptional activator protein Pur-alpha

Entrez Gene IDs

5813 (Human)

Gene Symbol

PURA

UniProt

NP_005850 (Human)

Additional PURA Products

Product Documents for PURA Antibody (3F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PURA Antibody (3F10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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