Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PURA Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human Purine-Rich Element Binding Protein Alpha using the numbering given in entry NP_005850.1 (GeneID 5813).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PURA Antibody - BSA Free

Western Blot: PURA Antibody [NBP1-92651]

Western Blot: PURA Antibody [NBP1-92651]

Western Blot: PURA Antibody [NBP1-92651] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pur-alpha antibody NBP1-92651 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Pur-alpha was more efficiently immunoprecipitated by rabbit anti-Pur-alpha antibody NBP1-92652, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Applications for PURA Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2000-1:10000
Application Notes
Immunoprecipitation Not recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PURA

PURA product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.

Alternate Names

PUR1PUR-ALPHA, PURALPHA, purine-rich element binding protein A, Purine-rich single-stranded DNA-binding protein alpha, transcriptional activator protein Pur-alpha

Gene Symbol

PURA

Additional PURA Products

Product Documents for PURA Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PURA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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