Key Product Details

Species Reactivity

Zebrafish

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # V11P4G11*E7

Format

BSA Free

Concentration

1.1 mg/ml

View all PXR/NR1I2 Antibodies »

Product Specifications

Immunogen

Ovalbumin-conjugated synthetic peptide EMRSMNEEYT (C-terminal sequence)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PXR/NR1I2 Antibody (V11P4G11*E7) - BSA Free

Western Blot: PXR/NR1I2 Antibody (V11P4G11*E7)BSA Free [NBP2-50519]

Western Blot: PXR/NR1I2 Antibody (V11P4G11*E7)BSA Free [NBP2-50519]

Western Blot: PXR/NR1I2 Antibody (V11P4G11/E7) [NBP2-50519] - Detection of PXR.

Immunohistochemistry: PXR/NR1I2 Antibody (V11P4G11/E7) [NBP2-50519] -

Immunohistochemistry: PXR/NR1I2 Antibody (V11P4G11/E7) [NBP2-50519] - Staining of PXR using clone V11P4G11 E7 on whole-mount zebrafish embryo

Applications for PXR/NR1I2 Antibody (V11P4G11*E7) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): ELISA - peptide immunogen; WB - whole organism lysate; IHC - zebrafish embryo.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PXR/NR1I2

NR1I2 product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.

Long Name

Pregnane X Receptor

Alternate Names

BXR, NR1I2, ONR1, PAR1, PAR2, SXR

Gene Symbol

NR1I2

Additional PXR/NR1I2 Products

Product Documents for PXR/NR1I2 Antibody (V11P4G11*E7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PXR/NR1I2 Antibody (V11P4G11*E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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