PXR/NR1I2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28799

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PXR/NR1I2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence EQFAITLKSYIECNR corresponding to internal region according to NP_003880.3, NP_071285.1, NP_148934.1.

Epitope

internal region

Specificity

This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PXR/NR1I2 Antibody

Western Blot: PXR/NR1I2 Antibody [NBP1-28799]

Western Blot: PXR/NR1I2 Antibody [NBP1-28799]

Western Blot: PXR/NR1I2 Antibody [NBP1-28799] - (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PXR/NR1I2 Antibody [NBP1-28799]

Immunohistochemistry-Paraffin: PXR/NR1I2 Antibody [NBP1-28799]

Immunohistochemistry-Paraffin: PXR/NR1I2 Antibody [NBP1-28799] - (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PXR/NR1I2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:4000

Western Blot

1-3 ug/ml
Application Notes
Western blot: Approx. 48kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8kDa according to NP_003880.3). An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Small Intestine.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PXR/NR1I2

This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq]

Long Name

Pregnane X Receptor

Alternate Names

BXR, NR1I2, ONR1, PAR1, PAR2, SXR

Entrez Gene IDs

8856 (Human)

Gene Symbol

NR1I2

UniProt

NP_003880.3 (Human)

Additional PXR/NR1I2 Products

Product Documents for PXR/NR1I2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PXR/NR1I2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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