PYCR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13834

Novus Biologicals, part of Bio-Techne
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NBP2-13834
NBP2-13834-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PYCR1 Antibodies »

Product Summary for PYCR1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KKTILDKDHLPLELGSPEGLHPLLLQYQLARAPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PYCR1 Antibody

Western Blot: PYCR1 Antibody [NBP2-13834]

Western Blot: PYCR1 Antibody [NBP2-13834]

Western Blot: PYCR1 Antibody [NBP2-13834] - Analysis in control (vector only transfected HEK293T lysate) and PYCR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PYCR1 Antibody [NBP2-13834]

Immunohistochemistry-Paraffin: PYCR1 Antibody [NBP2-13834]

Immunohistochemistry-Paraffin: PYCR1 Antibody [NBP2-13834] - Staining of human liver shows low positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: PYCR1 Antibody [NBP2-13834]

Immunohistochemistry-Paraffin: PYCR1 Antibody [NBP2-13834]

Immunohistochemistry-Paraffin: PYCR1 Antibody [NBP2-13834] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
View 1 more images

Applications for PYCR1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PYCR1

PYCR1 encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

ARCL2B, EC 1.5.1.2, mitochondrial pyrroline-5-carboxylate reductase 1, P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PRO3, proliferation-inducing protein 45, PYCR, pyrroline-5-carboxylate reductase 1, pyrroline-5-carboxylate reductase 1, mitochondrial

Gene Symbol

PYCR1

Additional PYCR1 Products

Product Documents for PYCR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PYCR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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