PYCRL Antibody (4F11)
Novus Biologicals, part of Bio-Techne | Catalog # H00065263-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4F11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PYCRL (NP_075566, 32 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVV
Reactivity Notes
Other species not tested.
Specificity
PYCRL (4F11)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for PYCRL Antibody (4F11)
Western Blot: PYCRL Antibody (4F11) [H00065263-M01]
Western Blot: PYCRL Antibody (4F11) [H00065263-M01] - PYCRL monoclonal antibody (M01), clone 4F11 Analysis of PYCRL expression in HeLa.Immunocytochemistry/ Immunofluorescence: PYCRL Antibody (4F11) [H00065263-M01]
Immunocytochemistry/Immunofluorescence: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of monoclonal antibody to PYCRL on HeLa cell. Antibody concentration 10 ug/ml.Western Blot: PYCRL Antibody (4F11) [H00065263-M01]
Western Blot: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of PYCRL expression in transfected 293T cell line by PYCRL monoclonal antibody (M01), clone 4F11.Lane 1: PYCRL transfected lysate (Predicted MW: 28.6 KDa).Lane 2: Non-transfected lysate.Applications for PYCRL Antibody (4F11)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PYCRL
Alternate Names
EC 1.5.1.2, FLJ13852, P5C reductase 3, P5CR 3, pyrroline-5-carboxylate reductase 3, pyrroline-5-carboxylate reductase-like, Pyrroline-5-carboxylate reductase-like protein
Entrez Gene IDs
65263 (Human)
Gene Symbol
PYCR3
UniProt
Additional PYCRL Products
Product Documents for PYCRL Antibody (4F11)
Product Specific Notices for PYCRL Antibody (4F11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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