Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PYGL Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 797 to 847 of human Phosphorylase, Glycogen, Liver using the numbering given in entry NP_002854.3 (GeneID 5836).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PYGL Antibody

Western Blot: PYGL Antibody [NBP2-32246]

Western Blot: PYGL Antibody [NBP2-32246]

Western Blot: PYGL Antibody [NBP2-32246] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PYGL antibody NBP2-32246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: PYGL Antibody [NBP2-32246]

Immunoprecipitation: PYGL Antibody [NBP2-32246]

Immunoprecipitation: PYGL Antibody [NBP2-32246] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from 293T cells. Antibodies: Affinity purified rabbit anti-PYGL antibody NBP2-32246 used for IP at 6 ug per reaction. PYGL was also immunoprecipitated by rabbit anti-PYGL antibody BL15846. For blotting immunoprecipitated PYGL, NBP2-32246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PYGL Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PYGL

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose 6 phosphate allosterically inhibit, phosphorylase B.

Long Name

Glycogen phosphorylase, liver form

Alternate Names

EC 2.4.1.1, glycogen phosphorylase, liver form, GSD6, phosphorylase, glycogen, liver, phosphorylase, glycogen; liver

Gene Symbol

PYGL

UniProt

NP_002854.3 (Human)

Additional PYGL Products

Product Documents for PYGL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PYGL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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