Pyridoxal Kinase/PDXK Antibody (4G6)

Novus Biologicals, part of Bio-Techne | Catalog # H00008566-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pyridoxal Kinase/PDXK Antibodies »

Product Specifications

Immunogen

PDXK (AAH00123, 1 a.a. ~ 312 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEECRVLSIQSHVIRGYVGNRAATFPLQVLGFEIDAVNSVQFSNHTGYAHWKGQVLNSDELQELYEGLRLNNMNKYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVLGDKWDGEGSMYVPEDLLPVYKEKVVPLADIITPNQFEAELLSGRKIHSQEEALRVMDMLHSMGPDTVVITSSDLPSPQGSNYLIVLGSQRRRNPAGSVVMERIRMDIRKVDAVFVGTGDLFAAMLLAWTHKHPNNLKVACEKTVSTLHHVLQRTIQCAKAQAGEGVRPSPMQLELRMVQSKRDIEDPEIVVQATVL

Specificity

Reacts with pyridoxal (pyridoxine, vitamin B6) kinase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Pyridoxal Kinase/PDXK Antibody (4G6)

Western Blot: Pyridoxal Kinase/PDXK Antibody (4G6) [H00008566-M03]

Western Blot: Pyridoxal Kinase/PDXK Antibody (4G6) [H00008566-M03]

Western Blot: Pyridoxal Kinase/PDXK Antibody (4G6) [H00008566-M03] - Analysis of PDXK expression in transfected 293T cell line by PDXK monoclonal antibody (M03), clone 4G6. Lane 1: PDXK transfected lysatE (35.1 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Pyridoxal Kinase/PDXK Antibody (4G6) [H00008566-M03] - Detection limit for recombinant GST tagged PDXK is 3 ng/ml as a capture antibody.

Applications for Pyridoxal Kinase/PDXK Antibody (4G6)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Pyridoxal Kinase/PDXK

The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Alternate Names

C21orf124, C21orf97, HEL-S-1a, PDXK, PKH, PNK, Vitamin B6 Kinase

Entrez Gene IDs

8566 (Human)

Gene Symbol

PDXK

UniProt

AAH00123 (Human)

Additional Pyridoxal Kinase/PDXK Products

Product Documents for Pyridoxal Kinase/PDXK Antibody (4G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Pyridoxal Kinase/PDXK Antibody (4G6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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