Pyridoxal Kinase/PDXK Antibody (OTI5H5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00821
![Flow Cytometry: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]](https://resources.bio-techne.com/images/products/PDXK-Antibody-5H5-Flow-Cytometry-NBP2-00821-img0001.jpg "Flow Cytometry: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]")
![Western Blot: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]](https://resources.bio-techne.com/images/products/PDXK-Antibody-5H5-Western-Blot-NBP2-00821-img0002.jpg "Western Blot: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI5H5
Concentration
0.82 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PDXK(NP_03672) produced in HEK293 cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
34.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pyridoxal Kinase/PDXK Antibody (OTI5H5)
Western Blot: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]
Western Blot: PDXK Antibody (5H5) [NBP2-00821] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDXK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDXK.
Immunocytochemistry/Immunofluorescence: PDXK Antibody (5H5) [NBP2-00821] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDXK.
![Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]](https://resources.bio-techne.com/images/products/PDXK-Antibody-5H5-Immunohistochemistry-Paraffin-NBP2-00821-img0008.jpg "Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]")
Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]
Immunohistochemistry-Paraffin: PDXK Antibody (5H5) [NBP2-00821] - Staining of paraffin-embedded Human pancreas tissue using anti-PDXK mouse monoclonal antibody.![Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]](https://resources.bio-techne.com/images/products/PDXK-Antibody-5H5-Immunohistochemistry-Paraffin-NBP2-00821-img0003.jpg "Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]")
![Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]](https://resources.bio-techne.com/images/products/PDXK-Antibody-5H5-Immunohistochemistry-Paraffin-NBP2-00821-img0004.jpg "Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]")
![Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]](https://resources.bio-techne.com/images/products/PDXK-Antibody-5H5-Immunohistochemistry-Paraffin-NBP2-00821-img0005.jpg "Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]")
![Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]](https://resources.bio-techne.com/images/products/PDXK-Antibody-5H5-Immunohistochemistry-Paraffin-NBP2-00821-img0006.jpg "Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]")
![Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]](https://resources.bio-techne.com/images/products/PDXK-Antibody-5H5-Immunohistochemistry-Paraffin-NBP2-00821-img0007.jpg "Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody (OTI5H5) [NBP2-00821]")
Applications for Pyridoxal Kinase/PDXK Antibody (OTI5H5)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Pyridoxal Kinase/PDXK
Alternate Names
C21orf124, C21orf97, HEL-S-1a, PDXK, PKH, PNK, Vitamin B6 Kinase
Entrez Gene IDs
8566 (Human)
Gene Symbol
PDXK
Additional Pyridoxal Kinase/PDXK Products
Product Documents for Pyridoxal Kinase/PDXK Antibody (OTI5H5)
Product Specific Notices for Pyridoxal Kinase/PDXK Antibody (OTI5H5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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