Pyridoxal Kinase/PDXK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88283

Novus Biologicals, part of Bio-Techne
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NBP1-88283
NBP1-88283-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pyridoxal Kinase/PDXK Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PNNLKVACEKTVSTLHHVLQRTIQCAKAQAGEGVRPSPMQLELRMVQSKRDIEDPEIVVQATVL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pyridoxal Kinase/PDXK Antibody

Western Blot: Pyridoxal Kinase/PDXK Antibody [NBP1-88283]

Western Blot: Pyridoxal Kinase/PDXK Antibody [NBP1-88283]

Western Blot: Pyridoxal Kinase/PDXK Antibody [NBP1-88283] - Analysis using Anti-PDXK antibody NBP1-88283 (A) shows similar pattern to independent antibody NBP1-88284 (B).
Immunocytochemistry/ Immunofluorescence: Pyridoxal Kinase/PDXK Antibody [NBP1-88283]

Immunocytochemistry/ Immunofluorescence: Pyridoxal Kinase/PDXK Antibody [NBP1-88283]

Immunocytochemistry/Immunofluorescence: Pyridoxal Kinase/PDXK Antibody [NBP1-88283] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody [NBP1-88283]

Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody [NBP1-88283]

Immunohistochemistry-Paraffin: Pyridoxal Kinase/PDXK Antibody [NBP1-88283] - Staining of human gall bladder shows moderate cytoplasmic and nuclear positivity in glandular cells.
View 1 more images

Applications for Pyridoxal Kinase/PDXK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyridoxal Kinase/PDXK

Pyridoxal Kinase/PDXK is encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Alternate Names

C21orf124, C21orf97, HEL-S-1a, PDXK, PKH, PNK, Vitamin B6 Kinase

Gene Symbol

PDXK

Additional Pyridoxal Kinase/PDXK Products

Product Documents for Pyridoxal Kinase/PDXK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pyridoxal Kinase/PDXK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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