Pyruvate Dehydrogenase E1 beta subunit Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38327

Novus Biologicals, part of Bio-Techne
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NBP2-38327
NBP2-38327-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase E1 beta subunit Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LIKSAIRDNNPVVVLENELMYGVPFEFPPEAQSKDFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pyruvate Dehydrogenase E1 beta subunit Antibody

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Analysis in human heart muscle and pancreas tissues. Corresponding Pyruvate Dehydrogenase E1 beta subunit RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Staining of human cerebral cortex, heart muscle, kidney and pancreas using Anti-Pyruvate Dehydrogenase E1 beta subunit antibody NBP2-38327 (A) shows similar protein distribution across tissues to independent antibody NBP1-87421 (B).
Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327]

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327]

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Analysis using Anti-PDHB antibody NBP2-38327 (A) shows similar pattern to independent antibody NBP1-87421 (B).
View 6 more images

Applications for Pyruvate Dehydrogenase E1 beta subunit Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

reported by internal validation

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western internal validation: Separated by size; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E1 beta subunit

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)

Alternate Names

DKFZp564K0164, mitochondrial, pyruvate dehydrogenase (lipoamide) beta, pyruvate dehydrogenase, E1 beta polypeptide

Gene Symbol

PDHB

UniProt

P11177 (Human)

Additional Pyruvate Dehydrogenase E1 beta subunit Products

Product Documents for Pyruvate Dehydrogenase E1 beta subunit Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pyruvate Dehydrogenase E1 beta subunit Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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