Pyruvate Dehydrogenase E2 Antibody (JE34-38)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32880
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE34-38
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within human Pyruvate Dehydrogenase E2 aa 574-624/647. (Uniprot: P10515)
Localization
Mitochondrion matrix.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody (JE34-38)
Western Blot: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880]
Western Blot: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880] - Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (NBP3-32880) at 1/500 dilution.Lane 1: SK-Br-3 cell lysate
Lane 2: K562 cell lysate
Lane 3: Daudi cell lysate
Lane 4: HL-60 cell lysate
Lysates/proteins at 10 ug/Lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32880) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880]
Immunohistochemistry: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (NBP3-32880) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32880) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for Pyruvate Dehydrogenase E2 Antibody (JE34-38)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase E2
Alternate Names
70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial
Gene Symbol
DLAT
Additional Pyruvate Dehydrogenase E2 Products
Product Documents for Pyruvate Dehydrogenase E2 Antibody (JE34-38)
Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody (JE34-38)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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