Pyruvate Dehydrogenase E2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99340
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]](https://resources.bio-techne.com/images/products/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP2-99340-img0005.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Pyruvate Dehydrogenase E2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDa![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]](https://resources.bio-techne.com/images/products/Pyruvate-Dehydrogenase-E2-Antibody-Immunohistochemistry-Paraffin-NBP2-99340-img0003.jpg "Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]")
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Immunochemical staining of human Pyruvate Dehydrogenase E2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.![Immunoprecipitation: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]](https://resources.bio-techne.com/images/products/Pyruvate-Dehydrogenase-E2-Antibody-Immunoprecipitation-NBP2-99340-img0004.jpg "Immunoprecipitation: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]")
Immunoprecipitation: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]
Immunoprecipitation: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Pyruvate Dehydrogenase E2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 ul anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDaApplications for Pyruvate Dehydrogenase E2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
0.5-2 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase E2
Alternate Names
70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial
Gene Symbol
DLAT
Additional Pyruvate Dehydrogenase E2 Products
Product Documents for Pyruvate Dehydrogenase E2 Antibody
Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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