Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16720
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5T6S8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 337-436 of human Pyruvate Dehydrogenase Kinase 1/PDK1 (NP_002601.1). STAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8) [NBP3-16720]](https://resources.bio-techne.com/images/products/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-9A7X4-Western-Blot-NBP3-16720-img0001.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8) [NBP3-16720]")
Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8) [NBP3-16720]
Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (9A7X4) [NBP3-16720] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase Kinase 1/PDK1/PDHK1 Rabbit mAb (NBP3-16720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Applications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8)
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Western Blot
1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase Kinase 1/PDK1
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.
Alternate Names
EC 2.7.11, EC 2.7.11.2, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, PDH kinase 1, PDK1, PDK-1, PKB kinase, Pyruvate dehydrogenase kinase isoform 1, pyruvate dehydrogenase kinase, isoenzyme 1, pyruvate dehydrogenase kinase, isozyme 1, Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial
Gene Symbol
PDK1
Additional Pyruvate Dehydrogenase Kinase 1/PDK1 Products
Product Documents for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8)
Product Specific Notices for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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