Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67032

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67032
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA67-30

Concentration

1 mg/ml

View all Pyruvate Dehydrogenase Kinase 1/PDK1 Antibodies »

Product Summary for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)

Immunogen

Synthetic peptide within Human Pyruvate Dehydrogenase Kinase 1/PDK1 aa 16-65 / 436. (SwissProt: Q15118 Human; SwissProt: Q8BFP9 Mouse; SwissProt: Q63065 Rat)

Localization

Mitochondrion matrix.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)

Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032]

Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032]

Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Western blot analysis of Pyruvate Dehydrogenase Kinase 1/PDK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysate
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032]

Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032]

Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Staining PDK1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of paraffin-embedded mouse heart tissue using anti-PDK1 antibody. Counter stained with hematoxylin.
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Applications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase Kinase 1/PDK1

Pyruvate dehydrogenase kinase isoform 1 (PDK1) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhbits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.

Alternate Names

EC 2.7.11, EC 2.7.11.2, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, PDH kinase 1, PDK1, PDK-1, PKB kinase, Pyruvate dehydrogenase kinase isoform 1, pyruvate dehydrogenase kinase, isoenzyme 1, pyruvate dehydrogenase kinase, isozyme 1, Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial

Gene Symbol

PDK1

Additional Pyruvate Dehydrogenase Kinase 1/PDK1 Products

Product Documents for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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