QKI/Quaking Antibody (GT1589)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13575

Novus Biologicals, part of Bio-Techne
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NBP3-13575
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (98%), Feline (100%), Porcine (100%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1589

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all QKI/Quaking Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human QKI/Quaking. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for QKI/Quaking Antibody (GT1589)

Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575]

Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575]

Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (GT1589) [NBP3-13575]

Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (GT1589) [NBP3-13575]

Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: QKI/Quaking protein stained by QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Frozen: QKI/Quaking Antibody (GT1589) [NBP3-13575]

Immunohistochemistry-Frozen: QKI/Quaking Antibody (GT1589) [NBP3-13575]

Immunohistochemistry-Frozen: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI/Quaking protein stained by QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
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Applications for QKI/Quaking Antibody (GT1589)

Application
Recommended Usage

Flow Cytometry

0.5ug/5x10^5cells~

Immunohistochemistry-Paraffin

5ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: QKI/Quaking

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Alternate Names

DKFZp586I0923, HKQ, homolog of mouse quaking QKI (KH domain RNA binding protein), Hqk, hqkI, protein quaking, QK, QK1, QK3, quaking homolog, KH domain RNA binding (mouse), RNA binding protein HQK

Gene Symbol

QKI

Additional QKI/Quaking Products

Product Documents for QKI/Quaking Antibody (GT1589)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for QKI/Quaking Antibody (GT1589)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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