Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all QKI/Quaking Antibodies »

Product Specifications

Immunogen

A synthetic peptide mapping to a region between residues 50 and 100 of isoform 5 of human quaking homolog using the numbering given in entry AAH19917.1 (GeneID 9444).

Specificity

The epitope recognized is common to isoforms 1, 2, 3, 4, 5, 6, 7 and 7B according to TrEMBL entries Q9P0Y1, Q9P0Y0, Q9P0X6, Q9P0X8, Q96PU8, Q96PU7, Q969L9 and Q96PU6, respectively.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for QKI/Quaking Antibody

Western Blot: QKI/Quaking Antibody [NB300-239]

Western Blot: QKI/Quaking Antibody [NB300-239]

Western Blot: QKI/Quaking Antibody [NB300-239] - Detection of Human QKI by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-QKI antibody NB300-239 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: QKI/Quaking Antibody [NB300-239]

Western Blot: QKI/Quaking Antibody [NB300-239]

Western Blot: QKI/Quaking Antibody [NB300-239] - Nuclear extract (20 ug) from HeLa cells. Antibody used at 0.2 ug/ml.

Applications for QKI/Quaking Antibody

Application
Recommended Usage

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: QKI/Quaking

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Alternate Names

DKFZp586I0923, HKQ, homolog of mouse quaking QKI (KH domain RNA binding protein), Hqk, hqkI, protein quaking, QK, QK1, QK3, quaking homolog, KH domain RNA binding (mouse), RNA binding protein HQK

Entrez Gene IDs

9444 (Human)

Gene Symbol

QKI

UniProt

Q96PU8 (Human)

Additional QKI/Quaking Products

Product Documents for QKI/Quaking Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for QKI/Quaking Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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