R-Spondin 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77354

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human R-Spondin 1. The immunogen is located within the first 50 amino acids of R-Spondin 1. Amino Acid Sequence: RGIKGKRQRRISAEGS

Specificity

At least three isoforms of R-Spondin 1 are known to exist; this antibody will detect the largest and smallest isoforms. R-Spondin 1 antibody will not cross-react with other RSPO family members. Human R-Spondin 1 has 3 isoforms, including isoform 1 (263aa, 29kD), isoform 2 (236aa, 26kD), and isoform 3 (200aa, 22kD). Mouse R-Spondin 1 had 1 isoform (265aa, 29kD) and rat R-Spondin 1 had 1 isoform (262aa, 29kD). NBP1-77354 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for R-Spondin 1 Antibody - BSA Free

Western Blot: R-Spondin 1 AntibodyBSA Free [NBP1-77354]

Western Blot: R-Spondin 1 AntibodyBSA Free [NBP1-77354]

Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 15 ug of spleen lysates per lane. Antibodies: RSPO1,5171 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL), 1h incubationat RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgGHRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: R-Spondin 1 Antibody - BSA Free [NBP1-77354]

Immunocytochemistry/ Immunofluorescence: R-Spondin 1 Antibody - BSA Free [NBP1-77354]

Immunocytochemistry/Immunofluorescence: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - R-Spondin 1 Antibody [NBP1-77354] - Staining of RSPO1 in human spleen tissue with RSPO1 antibody at 5 ug/ml.
Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354]

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354]

Immunohistochemistry: R-Spondin 1 Antibody [NBP1-77354] - RSPO1 in human spleen tissue with RSPO1 antibody at 5 ug/ml.
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Applications for R-Spondin 1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

2.5-5 ug/mL

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: R-Spondin 1

RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members.

Long Name

Roof Plate-specific Spondin 1

Alternate Names

Cristin 3, CRISTIN3, HRspo1, RSPO, RSPO1, RSpondin 1

Gene Symbol

RSPO1

UniProt

NP_001033722 (Human)

Additional R-Spondin 1 Products

Product Documents for R-Spondin 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for R-Spondin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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