RAB10 Antibody (PSH03-24)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32882
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH03-24
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within human RAB10 aa 101-150 / 200. (Uniprot: P61026)
Localization
Cytoplasmic vesicle membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RAB10 Antibody (PSH03-24)
Western Blot: RAB10 Antibody (PSH03-24) [NBP3-32882]
Western Blot: RAB10 Antibody (PSH03-24) [NBP3-32882] - Western blot analysis of RAB10 on different lysates with Rabbit anti-RAB10 antibody (NBP3-32882) at 1/2,000 dilution.Lane 1: A549 cell lysate
Lane 2: HeLa cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C6 cell lysate
Lane 8: Mouse brain tissue lysate
Lane 9: Rat brain tissue lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32882) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for RAB10 Antibody (PSH03-24)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RAB10
Alternate Names
GTP-binding protein RAB10, RAB10, member RAS oncogene family, ras-related GTP-binding protein, ras-related protein Rab-10
Gene Symbol
RAB10
Additional RAB10 Products
Product Documents for RAB10 Antibody (PSH03-24)
Product Specific Notices for RAB10 Antibody (PSH03-24)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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