RAB10 Antibody (PSH03-24)

Western Blot: RAB10 Antibody (PSH03-24) [NBP3-32882]

Western Blot: RAB10 Antibody (PSH03-24) [NBP3-32882] - Western blot analysis of RAB10 on different lysates with Rabbit anti-RAB10 antibody (NBP3-32882) at 1/2,000 dilution.

Lane 1: A549 cell lysate
Lane 2: HeLa cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C6 cell lysate
Lane 8: Mouse brain tissue lysate
Lane 9: Rat brain tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32882) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.