RAB11B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18858
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human RAB11B (Accession#: ABQ59034.1; Gly2-Cys215)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
Scientific Data Images for RAB11B Antibody
Western Blot-NBP3-18858-RAB11B Antibody-
Western Blot-NBP3-18858-RAB11B Antibody-Lane A: 293T Whole Cell LysateLane B: U87-MG Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: SH-SY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa
ICC/IF-NBP3-18858-RAB11B Antibody-
ICC/IF-NBP3-18858-RAB11B Antibody- Staining of S1h-3C-RAB11B in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human S1h-3C-RAB11B polyclonal antibody (dilution ratio 1:100) at 4c overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunoprecipitation-NBP3-18858-RAB11B Antibody-
Immunoprecipitation-NBP3-18858-RAB11B Antibody-Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg U87-MG Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell LysateLane D:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-RAB11B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RAB11B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :24 kDaApplications for RAB11B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunoprecipitation
1- 5 uL/mg lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RAB11B
Alternate Names
GTP-binding protein YPT3, H-YPT3, MGC133246, RAB11B, member of RAS oncogene family, RAB11B, member RAS oncogene family, ras-related protein Rab-11B, YPT3
Gene Symbol
RAB11B
Additional RAB11B Products
Product Documents for RAB11B Antibody
Product Specific Notices for RAB11B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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