Rab27a Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03369
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human Rab27a (NP_899057.1). MSDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGIDFREKRVVYRASGPDGATGRGQRIHLQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYFETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGACGC
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rab27a Antibody - BSA Free
Knockdown Validated: Rab27a Antibody [NBP3-03369] -
Knockdown Validated: Rab27a Antibody [NBP3-03369] - Analysis of extracts from wild type(WT) and RAB27A knockdown (KD) U-87MG (KD) cells, using RAB27A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 10s.
Immunocytochemistry/Immunofluorescence: Rab27a Antibody [NBP3-03369] -
Immunocytochemistry/Immunofluorescence: Rab27a Antibody [NBP3-03369] -Analysis of HepG2 cells using [KD Validated] RAB27A Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Rab27a Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rab27a
Long Name
RAs Genes from Brain Protein 27A
Alternate Names
GS2, HsT18676, RAM
Gene Symbol
RAB27A
Additional Rab27a Products
Product Documents for Rab27a Antibody - BSA Free
Product Specific Notices for Rab27a Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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