RAB31 Antibody (1C6)

Western Blot: RAB31 Antibody (1C6) [H00011031-M01]

Western Blot: RAB31 Antibody (1C6) [H00011031-M01] - Rab31 expression levels in stably transfected breast cancer cells. pAb RT3-IgG specifically detects rab31 in cell lysates from stably transfected MDA-MB-231 and CAMA-1 breast cancer cells in Western blot analyses. Vector control, MDA-MB-231, or CAMA-1 cells were stably transfected with the empty vector pRcRSV; batch, parental MDA-MB-231 (low endogenous rab31 expression) or CAMA-1 (no endogenous rab31 expression) cells were stably transfected with the pRcRSV-based rab31 expression vector; high, medium or low, selected cell clones of batch-transfected MDA-MB-231 or CAMA-1 cells with differing high, medium or low protein levels of rab31. Image collected and cropped by CiteAb from the following publication (molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-11-62), licensed under a CC-BY license.

Western Blot: RAB31 Antibody (1C6) [H00011031-M01]

Western Blot: RAB31 Antibody (1C6) [H00011031-M01] - Western Blot detection against Immunogen (36.63 KDa) .

Western Blot: RAB31 Antibody (1C6) [H00011031-M01] -

Western Blot: RAB31 Antibody (1C6) [H00011031-M01] - Characterization of polyclonal antibodies directed to human rab31. (A) The reaction pattern of polyclonal antibody (pAb) RT3-IgG (black) & of the pre-immune rabbit serum (gray) was analyzed by a ‘one-sided ELISA’ assay [19]. rab31-His, purified recombinant, histidine-tagged rab31, used for immunization; GST-rab31, purified recombinant GST-rab31 fusion protein; BSA, bovine serum albumin. (B) In Western blot analyses, pAb RT3-IgG strongly reacts with recombinant rab31 & not at all or only weakly with the highly homologous Rab proteins rab5 & rab22A, respectively. (C) Immunohistochemical staining of paraffin-embedded, formalin-fixed breast cancer specimens with pAb RT4-IgG. Specific immunostaining is observed in the cytoplasm as well as in the nucleus of cancer cells (upper panel, see arrow), stromal cells are stained less frequently (lower panel, see arrow). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22920728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.