RAB35 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04511
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RAB35 Antibody - Azide and BSA Free
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-201 of human RAB35 (NP_006852.1). KRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RAB35 Antibody - Azide and BSA Free
Western Blot: RAB35 AntibodyAzide and BSA Free [NBP3-04511]
Western Blot: RAB35 Antibody [NBP3-04511] - Western blot analysis of extracts of various cell lines, using RAB35 antibody (NBP3-04511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Immunocytochemistry/ Immunofluorescence: RAB35 Antibody - Azide and BSA Free [NBP3-04511]
Immunocytochemistry/Immunofluorescence: RAB35 Antibody [NBP3-04511] - Immunofluorescence analysis of PC-12 cells using RAB35 Rabbit pAb (NBP3-04511) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: RAB35 Antibody - Azide and BSA Free [NBP3-04511]
Immunohistochemistry-Paraffin: RAB35 Antibody [NBP3-04511] - Immunohistochemistry of paraffin-embedded rat heart using RAB35 Rabbit pAb (NBP3-04511) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for RAB35 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RAB35
Alternate Names
GTP-binding protein RAY, H-ray, RAB1C, RAB35, member RAS oncogene family, Ras-related protein Rab-1C, ras-related protein rab-1c (GTP-binding protein ray), ras-related protein Rab-35, RAY
Gene Symbol
RAB35
Additional RAB35 Products
Product Documents for RAB35 Antibody - Azide and BSA Free
Product Specific Notices for RAB35 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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