Rab3A Antibody (4H7)

Novus Biologicals, part of Bio-Techne | Catalog # H00005864-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4H7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rab3A Antibodies »

Product Specifications

Immunogen

RAB3A (NP_002857, 122 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TYSWDNAQVLLVGNKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQGPQLSDQQVPPHQDCAC

Specificity

RAB3A - RAB3A, member RAS oncogene family

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Rab3A Antibody (4H7)

Western Blot: Rab3A Antibody (4H7) [H00005864-M01]

Western Blot: Rab3A Antibody (4H7) [H00005864-M01]

Western Blot: Rab3A Antibody (4H7) [H00005864-M01] - RAB3A monoclonal antibody (M01), clone 4H7 Analysis of RAB3A expression in IMR-32.
Immunocytochemistry/ Immunofluorescence: Rab3A Antibody (4H7) [H00005864-M01]

Immunocytochemistry/ Immunofluorescence: Rab3A Antibody (4H7) [H00005864-M01]

Immunocytochemistry/Immunofluorescence: Rab3A Antibody (4H7) [H00005864-M01] - Analysis of monoclonal antibody to RAB3A on HeLa cell. Antibody concentration 25 ug/ml.
Sandwich ELISA: Rab3A Antibody (4H7) [H00005864-M01] - Detection limit for recombinant GST tagged RAB3A is approximately 0.03ng/ml as a capture antibody.

Applications for Rab3A Antibody (4H7)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. IHC-P usage was reported in scientific literature.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rab3A

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D (Schluter et al., 2004). These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters (Coppola et al., 2002). Specifically, Rab3's are thought to play a key role in presynaptic vesicle trafficking and priming (Schluter et al., 2006).

Alternate Names

RAB3A, member RAS oncogene family, RAS-associated protein RAB3A, ras-related protein Rab-3A

Entrez Gene IDs

5864 (Human)

Gene Symbol

RAB3A

OMIM

179490 (Human)

UniProt

NP_002857 (Human)

Additional Rab3A Products

Product Documents for Rab3A Antibody (4H7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rab3A Antibody (4H7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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