Rab4 Antibody (4E11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37485

Novus Biologicals, part of Bio-Techne
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NBP2-37485
NBP2-37485-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse, Monkey, Primate

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E11

Format

BSA Free

Concentration

1 mg/ml

View all Rab4 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Rab4 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rab4 Antibody (4E11) - BSA Free

Western Blot: Rab4 Antibody (4E11)BSA Free [NBP2-37485]

Western Blot: Rab4 Antibody (4E11)BSA Free [NBP2-37485]

Western Blot: Rab4 Antibody (4E11) [NBP2-37485] - Analysis using RAB4A mouse mAb against Jurkat (1), HeLa (2), A549 (3), HEK293 (4), K562 (5), NIH3T3 (6), PC-12 (7), and COS7 (8) cell lysate.
Immunohistochemistry-Paraffin: Rab4 Antibody (4E11) - BSA Free [NBP2-37485]

Immunohistochemistry-Paraffin: Rab4 Antibody (4E11) - BSA Free [NBP2-37485]

Immunohistochemistry-Paraffin: Rab4 Antibody (4E11) [NBP2-37485] - Analysis of liver cancer tissues using RAB4A mouse mAb with DAB staining.
Flow Cytometry: Rab4 Antibody (4E11) - BSA Free [NBP2-37485]

Flow Cytometry: Rab4 Antibody (4E11) - BSA Free [NBP2-37485]

Flow Cytometry: Rab4 Antibody (4E11) [NBP2-37485] - Analysis of MCF-7 cells using RAB4A mouse mAb (green) and negative control (red).
View 3 more images

Applications for Rab4 Antibody (4E11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab4

Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Alternate Names

Oncogene RAB4, RAB4, member RAS oncogene family, RAB4A, member RAS oncogene family, RAB4HRES-1/RAB4, ras-related protein Rab-4A

Gene Symbol

RAB4A

Additional Rab4 Products

Product Documents for Rab4 Antibody (4E11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rab4 Antibody (4E11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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