Rab5a Antibody (3A4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04340
![Western Blot: Rab5a Antibody (3A4)BSA Free [NBP1-04340]](https://resources.bio-techne.com/images/products/Rab5a-Antibody-3A4-Western-Blot-NBP1-04340-img0001.jpg "Western Blot: Rab5a Antibody (3A4)BSA Free [NBP1-04340]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 3A4
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for Rab5a Antibody (3A4) - BSA Free
Immunogen
Recombinant human Rab5a (1-215aa) purified from E. coli
Marker
Early Endosome Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Rab5a Antibody (3A4) - BSA Free
Western Blot: Rab5a Antibody (3A4)BSA Free [NBP1-04340]
Western Blot: Rab5a Antibody (3A4) [NBP1-04340] - The extracts of mouse brain (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Flow Cytometry: Mouse Rab5a mAb (3A4) - BSA Free [NBP1-04340] -
Flow Cytometry: Mouse Rab5a mAb (3A4) - BSA Free [NBP1-04340] - Flow cytometry of purified endosomes from mouse bone marrow derived dendritic cells bearing (blue curve) or absent of (red curve) fluorescent antigen. Endosomes stained with Alex Fluor 700 conjugated to Rab5a (NBP1-04340AF700). Image from a verified customer review.
Detection of Rab5a by Immunoprecipitation.
HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of Rab5a antibody (Catalog # NBP1-04340) pre-coupled to Dynabeads protein G. Samples were washed and processed for Western Blot with Rab5a antibody. For Western Blot, Rb x Rab5a was used at 1/1000. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).Applications for Rab5a Antibody (3A4) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
Validated for Flow Cytometry from a verified customer review.
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rab5a
Alternate Names
RAB5A, member RAS oncogene family, RAB5RAS-associated protein RAB5A, ras-related protein Rab-5A
Gene Symbol
RAB5A
UniProt
Additional Rab5a Products
Product Documents for Rab5a Antibody (3A4) - BSA Free
Product Specific Notices for Rab5a Antibody (3A4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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