RAB6A Antibody (HL1047)

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701]

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

ICC/IF-RAB6A Antibody (HL1047) [NBP3-13701] -

ICC/IF-RAB6A Antibody (HL1047) [NBP3-13701] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB6A stained by RAB6A antibody [HL1047] diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] -

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] -Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] -

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.

Western Blot Shows Human RAB6A Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 cell line and RAB6A knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with RAB6A Antibody (HL1047) (Catalog # NBP3-13701) followed by HRP-conjugated secondary antibody. A specific band was detected for RAB6A at approximately 24 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/200. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.