Rab7a Antibody (10E4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60237
![Western Blot: Rab7a Antibody (10E4)BSA Free [NBP2-60237]](https://resources.bio-techne.com/images/products/Rab7a-Antibody-10E4-Western-Blot-NBP2-60237-img0001.jpg "Western Blot: Rab7a Antibody (10E4)BSA Free [NBP2-60237]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 10E4
Format
BSA Free
Concentration
1 mg/ml
Product Summary for Rab7a Antibody (10E4) - BSA Free
Immunogen
Recombinant human RAB7A (1-207aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Rab7a Antibody (10E4) - BSA Free
Western Blot: Rab7a Antibody (10E4)BSA Free [NBP2-60237]
Western Blot: Rab7a Antibody (10E4) [NBP2-60237] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab7A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysate Lane 3.: MCF7 cell lysate Lane 4.: PC3 cell lysateLane 5.: 293T cell lysateLane 6.: SK-OV-3 cell lysate![Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (10E4) - BSA Free [NBP2-60237]](https://resources.bio-techne.com/images/products/Rab7a-Antibody-10E4-Immunocytochemistry-Immunofluorescence-NBP2-60237-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (10E4) - BSA Free [NBP2-60237]")
Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (10E4) - BSA Free [NBP2-60237]
Immunocytochemistry/Immunofluorescence: Rab7a Antibody (10E4) [NBP2-60237] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).![Flow Cytometry: Rab7a Antibody (10E4) - BSA Free [NBP2-60237]](https://resources.bio-techne.com/images/products/Rab7a-Antibody-10E4-Flow-Cytometry-NBP2-60237-img0004.jpg "Flow Cytometry: Rab7a Antibody (10E4) - BSA Free [NBP2-60237]")
Flow Cytometry: Rab7a Antibody (10E4) - BSA Free [NBP2-60237]
Flow Cytometry: Rab7a Antibody (10E4) [NBP2-60237] - Analysis of RAB7A in HeLa cells. The cell was stained with at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotypecontrol (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Applications for Rab7a Antibody (10E4) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
2-5 ug
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rab7a
Long Name
RAs Genes from Brain Protein 7a
Alternate Names
PRO2706, RAB7
Gene Symbol
RAB7A
Additional Rab7a Products
Product Documents for Rab7a Antibody (10E4) - BSA Free
Product Specific Notices for Rab7a Antibody (10E4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...