Rab7a Antibody (5A10A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15444

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15444-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5A10A4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Rab7a Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 108-207 of human Rab7a (NP_004628.4). IQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab7a Antibody (5A10A4)

Western Blot: Rab7a Antibody (5A10A4) [NBP3-15444]

Western Blot: Rab7a Antibody (5A10A4) [NBP3-15444]

Western Blot: Rab7a Antibody (4X3P6) [NBP3-15444] - Western blot analysis of extracts of various cell lines, using Rab7a Rabbit mAb (NBP3-15444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (5A10A4) [NBP3-15444]

Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (5A10A4) [NBP3-15444]

Immunocytochemistry/Immunofluorescence: Rab7a Antibody (4X3P6) [NBP3-15444] - Immunofluorescence analysis of NIH-3T3 cells using Rab7a Rabbit mAb (NBP3-15444) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Rab7a Antibody (5A10A4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rab7a

RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

Long Name

RAs Genes from Brain Protein 7a

Alternate Names

PRO2706, RAB7

Gene Symbol

RAB7A

Additional Rab7a Products

Product Documents for Rab7a Antibody (5A10A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rab7a Antibody (5A10A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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