RAB8A Antibody (7G8B11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52548

Novus Biologicals, part of Bio-Techne
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NBP2-52548
NBP2-52548-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 7G8B11

Format

BSA Free

Concentration

1 mg/ml

View all RAB8A Antibodies »

Product Summary for RAB8A Antibody (7G8B11) - BSA Free

Immunogen

Purified recombinant fragment of human RAB8A (AA: 68-207) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAB8A Antibody (7G8B11) - BSA Free

Western Blot: RAB8A Antibody (7G8B11)BSA Free [NBP2-52548]

Western Blot: RAB8A Antibody (7G8B11)BSA Free [NBP2-52548]

Western Blot: RAB8A Antibody (7G8B11) [NBP2-52548] - Analysis using Rab8 mAb against human Rab8 (AA: 68-207) recombinant protein. (Expected MW is 42 kDa)
Flow Cytometry: RAB8A Antibody (7G8B11) - BSA Free [NBP2-52548]

Flow Cytometry: RAB8A Antibody (7G8B11) - BSA Free [NBP2-52548]

Flow Cytometry: RAB8A Antibody (7G8B11) [NBP2-52548] - Analysis of HeLa cells using Rab8 mouse mAb (green) and negative control (red).
Western Blot: RAB8A Antibody (7G8B11)BSA Free [NBP2-52548]

Western Blot: RAB8A Antibody (7G8B11)BSA Free [NBP2-52548]

Western Blot: RAB8A Antibody (7G8B11) [NBP2-52548] - Analysis using Rab8 mAb against HEK293 (1) and Rab8 (AA: 68-207)-hIgGFc transfected HEK293 (2) cell lysate.
View 1 more images

Applications for RAB8A Antibody (7G8B11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAB8A

RAB8A is encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.

Long Name

Ras-related protein Rab-8A

Alternate Names

MEL, Oncogene c-mel, RAB8

Gene Symbol

RAB8A

Additional RAB8A Products

Product Documents for RAB8A Antibody (7G8B11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAB8A Antibody (7G8B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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