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RAB9A Antibody (7O1G4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16506

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16506-100ul
NBP3-16506-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7O1G4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAB9A (NP_004242.1). MAGKSSLFKVILLGDGGVGKSSLMNRYVTNKFDTQLFHTIGVEFLNKDLEVDGHFVTMQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDSQSFQNLSN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAB9A Antibody (7O1G4)

Western Blot: RAB9A Antibody (7O1G4) [NBP3-16506]

Western Blot: RAB9A Antibody (7O1G4) [NBP3-16506]

Western Blot: RAB9A Antibody (7O1G4) [NBP3-16506] - Western blot analysis of extracts of various cell lines, using RAB9A Rabbit mAb (NBP3-16506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (7O1G4) [NBP3-16506]

Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (7O1G4) [NBP3-16506]

Immunocytochemistry/Immunofluorescence: RAB9A Antibody (7O1G4) [NBP3-16506] - Immunofluorescence analysis of NIH-3T3 cells using RAB9A Rabbit mAb (NBP3-16506) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RAB9A Antibody (7O1G4) [NBP3-16506]

Immunohistochemistry-Paraffin: RAB9A Antibody (7O1G4) [NBP3-16506]

Immunohistochemistry-Paraffin: RAB9A Antibody (7O1G4) [NBP3-16506] - Immunohistochemistry of paraffin-embedded rat testis using RAB9A Rabbit mAb (NBP3-16506) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for RAB9A Antibody (7O1G4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAB9A

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Alternate Names

RAB9A, member RAS oncogene family, RAB9RAB9, member RAS oncogene family, ras-related protein Rab-9A

Gene Symbol

RAB9A

Additional RAB9A Products

Product Documents for RAB9A Antibody (7O1G4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RAB9A Antibody (7O1G4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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