Rac1,2,3 Antibody (AT2G10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88839
![Western Blot: Rac1,2,3 Antibody (AT2G10)BSA Free [NBP2-88839]](https://resources.bio-techne.com/images/products/Antibody-AT2G10-Western-Blot-NBP2-88839-img0002.jpg "Western Blot: Rac1,2,3 Antibody (AT2G10)BSA Free [NBP2-88839]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # AT2G10
Format
BSA Free
Concentration
1 mg/ml
Product Summary for Rac1,2,3 Antibody (AT2G10) - BSA Free
Immunogen
Recombinant human Rac1,2,3 (1-189aa) protein purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Rac1,2,3 Antibody (AT2G10) - BSA Free
Western Blot: Rac1,2,3 Antibody (AT2G10)BSA Free [NBP2-88839]
Western Blot: Antibody (AT2G10) [NBP2-88839] - The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. Jurkat cell lysate![Flow Cytometry: Rac1,2,3 Antibody (AT2G10) - BSA Free [NBP2-88839]](https://resources.bio-techne.com/images/products/Antibody-AT2G10-Flow-Cytometry-NBP2-88839-img0003.jpg "Flow Cytometry: Rac1,2,3 Antibody (AT2G10) - BSA Free [NBP2-88839]")
Flow Cytometry: Rac1,2,3 Antibody (AT2G10) - BSA Free [NBP2-88839]
Flow Cytometry: Antibody (AT2G10) [NBP2-88839] - Flow cytometry analysis of Rac1,2,3 in Jurkat cells. The cell was stained with at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).![Western Blot: Rac1,2,3 Antibody (AT2G10)BSA Free [NBP2-88839]](https://resources.bio-techne.com/images/products/Antibody-AT2G10-Western-Blot-NBP2-88839-img0001.jpg "Western Blot: Rac1,2,3 Antibody (AT2G10)BSA Free [NBP2-88839]")
Western Blot: Rac1,2,3 Antibody (AT2G10)BSA Free [NBP2-88839]
Western Blot: Antibody (AT2G10) [NBP2-88839] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human Rac 1 proteinLane 2.: Recombinant human Rac 2 proteinLane 3.: Recombinant human Rac 3 proteinApplications for Rac1,2,3 Antibody (AT2G10) - BSA Free
Application
Recommended Usage
Flow Cytometry
2-5ug
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rac1,2,3
Alternate Names
EN-7, Gx, HSPC022, MIG5, p21-Rac1, p21-Rac2, p21-Rac3, Rac-1, ras-related C3 botulinum toxin substrate 3, rho family, small GTP binding protein Rac3, TC-25
Gene Symbol
RAC1
Additional Rac1,2,3 Products
Product Documents for Rac1,2,3 Antibody (AT2G10) - BSA Free
Product Specific Notices for Rac1,2,3 Antibody (AT2G10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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