Rac1,2,3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32887
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein within Human RAC3 aa 23-192 / 192. (Uniprot: P15153)
Localization
Cytoplasm, Cytoplasm, Cell membrane.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rac1,2,3 Antibody
Western Blot: Rac1,2,3 Antibody [NBP3-32887]
Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution.Lane 1: Rat brain tissue lysate
Lane 2: Human brain tissue lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 5 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: Rac1,2,3 Antibody [NBP3-32887]
Immunohistochemistry: Rac1,2,3 Antibody [NBP3-32887] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/600 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32887) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: Rac1,2,3 Antibody [NBP3-32887]
Immunocytochemistry/ Immunofluorescence: Rac1,2,3 Antibody [NBP3-32887] - Immunocytochemistry analysis of SH-SY5Y cells labeling Rac1,2,3 with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Applications for Rac1,2,3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry-Paraffin
1:600
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rac1,2,3
Alternate Names
EN-7, Gx, HSPC022, MIG5, p21-Rac1, p21-Rac2, p21-Rac3, Rac-1, ras-related C3 botulinum toxin substrate 3, rho family, small GTP binding protein Rac3, TC-25
Gene Symbol
RAC1
Additional Rac1,2,3 Products
Product Documents for Rac1,2,3 Antibody
Product Specific Notices for Rac1,2,3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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