RAC3 Antibody (JE56-48)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32888
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE56-48
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human RAC3 aa 71-170 / 192. (Uniprot: P60763)
Localization
Cytoplasm, Endomembrane system, Cell projection, lamellipodium, Cytoplasm, perinuclear region, Cell membrane, cytoskeleton.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RAC3 Antibody (JE56-48)
Western Blot: RAC3 Antibody (JE56-48) [NBP3-32888]
Western Blot: RAC3 Antibody (JE56-48) [NBP3-32888] - Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (NBP3-32888) at 1/2,000 dilution.Lane 1: HEK-293 cell lysate (20 ug/Lane)
Lane 2: JAR cell lysate (20 ug/Lane)
Lane 3: HUVEC cell lysate (20 ug/Lane)
Lane 4: PC-12 cell lysate (20 ug/Lane)
Lane 5: SH-SY5Y cell lysate (15 ug/Lane)
Lane 6: C2C12 cell lysate (15 ug/Lane)
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32888) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for RAC3 Antibody (JE56-48)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RAC3
Long Name
Ras-related C3 botulinum toxin substrate 3
Alternate Names
p21-Rac3
Gene Symbol
RAC3
Additional RAC3 Products
Product Documents for RAC3 Antibody (JE56-48)
Product Specific Notices for RAC3 Antibody (JE56-48)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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