RAD18 Antibody (79B1048.1) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33217

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 79B1048.1

Format

Azide Free

Concentration

1.0 mg/ml

View all RAD18 Antibodies »

Product Specifications

Immunogen

The antibody was raised against a synthetic peptide corresponding to the amino acids 402-414 of human Rad18.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RAD18 Antibody (79B1048.1) - Azide Free

Western Blot: RAD18 Antibody (79B1048.1)Azide Free [NBP2-33217]

Western Blot: RAD18 Antibody (79B1048.1)Azide Free [NBP2-33217]

Western Blot: RAD18 Antibody (79B1048.1) - Azide Free [NBP2-33217] - The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate. Image from the standard format of this antibody.

Applications for RAD18 Antibody (79B1048.1) - Azide Free

Application
Recommended Usage

Western Blot

2-4 ug/ml
Application Notes
In HL60, a 54 kDa band should be detected.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin. Does not contain BSA

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAD18

RAD18 is encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Alternate Names

EC 6.3.2.-, hHR18, hRAD18, postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD18 homolog (S. cerevisiae), RAD18, S. cerevisiae, homolog, RING finger protein 73, RNF73E3 ubiquitin-protein ligase RAD18

Gene Symbol

RAD18

Additional RAD18 Products

Product Documents for RAD18 Antibody (79B1048.1) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAD18 Antibody (79B1048.1) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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