RAD18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61063

Novus Biologicals, part of Bio-Techne
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NB100-61063
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all RAD18 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 445 and 495 of human E3 ubiquitin-protein ligase RAD18 using the numbering given in entry NP_064550.2 (GeneID 56852).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAD18 Antibody

Western Blot: RAD18 Antibody [NB100-61063]

Western Blot: RAD18 Antibody [NB100-61063]

Western Blot: RAD18 Antibody [NB100-61063] - Detection of Human RAD18 on HeLa whole cell lysate using NB100-61063. RAD18 was also immunoprecipitated by rabbit anti-RAD18 antibody NB100-61062.

Applications for RAD18 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
KO Validation was reported in scientific literature (PMID: 27146073).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RAD18

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Alternate Names

EC 6.3.2.-, hHR18, hRAD18, postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD18 homolog (S. cerevisiae), RAD18, S. cerevisiae, homolog, RING finger protein 73, RNF73E3 ubiquitin-protein ligase RAD18

Entrez Gene IDs

56852 (Human)

Gene Symbol

RAD18

UniProt

Q9NS91 (Human)

Additional RAD18 Products

Product Documents for RAD18 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RAD18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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