RAD18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33865

Novus Biologicals, part of Bio-Techne
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NBP2-33865
NBP2-33865-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAD18 Antibodies »

Product Summary for RAD18 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LESPAKSPASSSSKNLAVKVYTPVASRQSLKQGSRLMDNFLIREMSGSTSELLIKENKSKFSPQKEASPAAKTKETRSVEEIAPDPSEAKRPEPPSTSTLKQVTKVDCP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAD18 Antibody

Western Blot: RAD18 Antibody [NBP2-33865]

Western Blot: RAD18 Antibody [NBP2-33865]

Western Blot: RAD18 Antibody [NBP2-33865] - Analysis in control (vector only transfected HEK293T lysate) and RAD18 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RAD18 Antibody [NBP2-33865]

Immunocytochemistry/ Immunofluorescence: RAD18 Antibody [NBP2-33865]

Immunocytochemistry/Immunofluorescence: RAD18 Antibody [NBP2-33865] - Staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.
Immunohistochemistry: RAD18 Antibody [NBP2-33865]

Immunohistochemistry: RAD18 Antibody [NBP2-33865]

Immunohistochemistry: RAD18 Antibody [NBP2-33865] - Staining of human lymph node shows distinct nuclear positivity in germinal center cells.

Applications for RAD18 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAD18

RAD18 is encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Alternate Names

EC 6.3.2.-, hHR18, hRAD18, postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD18 homolog (S. cerevisiae), RAD18, S. cerevisiae, homolog, RING finger protein 73, RNF73E3 ubiquitin-protein ligase RAD18

Gene Symbol

RAD18

UniProt

Q9NS91 (Human)

Additional RAD18 Products

Product Documents for RAD18 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAD18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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